Composite

Part:BBa_K2088692

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-20)


gene cluster mhbDHIM

This gene cluster encodes the enzymes involved in the catabolism of 3-hydroxybenzoate to fumarate and pyruvate via the gentisate pathway. mhbD, mhbH, mhbI and mhbM genes encode the gentisate 1,2-dioxygenase, maleylpyruvate isomerase, fumarylpyruvate hydratase and 3-HBA 6-hydroxylase respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2195
    Illegal BamHI site found at 2581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 431
    Illegal NgoMIV site found at 2385
    Illegal NgoMIV site found at 3522
    Illegal AgeI site found at 493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2715


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Categories
Parameters
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